Human DNA ligases in replication and repair
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E3 ubiquitin ligases: styles, structures and functions - PMC
The human DNA ligases are members of the nucleotidyl transferase family that also includes mRNA capping enzymes and RNA ligases (3,4).Nucleotidyl transferases contain a conserved NTD-OBD catalytic core that interacts with a nucleotide co-factor to form a covalent enzyme-nucleoside monophosphate intermediate in which the nucleoside monophosphate is linked via a phosphoramidite bond to a ...
DNA Ligases: Progress and Prospects - PMC - National Center for ...
Ubiquitin and its associated enzymes act as cellular guardians to ensure precise spatio-temporal control of key molecules during each of these important cellular processes. Loss of precision ...
Ligase - an overview | ScienceDirect Topics
In this review, we summarize the current progress in structure-function studies of ubiquitin ligases as well as exciting new discoveries of novel classes of E3s and diverse substrate recognition mechanisms. Our increased understanding of ubiquitin ligase function and regulation has provided the rationale for developing E3-targeting therapeutics ...
Ligation and Ligases - CSH Protocols
Key points: Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs. However, some produce blunt ends. DNA ligase is a DNA-joining enzyme.
Liga Sensa - Facebook
DNA ligase of E. coli is a single polypeptide of molecular weight 75,000. The comparable T4-induced enzyme is somewhat smaller (63,000 to 68,000). Both enzymes catalyze the synthesis of phosphodiester bonds between adjacent 5′-phosphoryl and 3′-hydroxyl groups in nicked duplex DNA, coupled to the cleavage of the pyrophosphate bond of DPN ...
What is DNA ligase? | IDT
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary ...
Restriction enzymes & DNA ligase (article) | Khan Academy
Ligases are enzymes that are capable of catalyzing the reaction of joining two large molecules by establishing a new chemical bond, generally with concomitant hydrolysis of a small chemical group on one of the bulky molecules or simply linking of two compounds together (e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
DNA Ligase: Structure, Mechanism, and Function | Science
Kokumi-active γ-glutamyl dipeptides accumulate during sourdough fermentation. γ-Glutamylcysteine ligases (Gcls) of Limosilactobacillus reuteri synthesize γ-glutamyl dipeptides during growth in sourdough. This study aimed to evaluate the contribution of Gcls from strains of L. reuteri in the formation of kokumi-active γ-glutamyl dipeptides in sourdough bread. Among 12 acceptor amino acids ...
Ubiquitin Ligases: Structure, Function, and Regulation
Ligases. Wolfgang Goedecke, in xPharm: The Comprehensive Pharmacology Reference, 2007. Introduction. The term ligase is used for a variety of entirely unrelated enzymes. The common feature of all of them is their ability to covalently link two substrate molecules together. Here, the term will be used for DNA-ligases, which play a central role in DNA replication, recombination, and repair.